An autoclave is a sterilization instrument that uses high-pressure steam to destroy microorganisms. Operating typically at 121°C and 15 psi for 15–20 minutes, it ensures complete sterilization. It is effective against bacteria, viruses, fungi, and resistant spores. Common applications include sterilizing surgical instruments, glassware, and culture media. The process is based on downward displacement of air by steam for thorough exposure. Autoclaves are crucial in maintaining aseptic conditions in laboratories, hospitals, and research centers.
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Sterilizes equipment using steam under pressure.
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Standard setting: 121°C at 15 psi for 15–20 minutes.
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Kills microorganisms including spores.
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Used for glassware, surgical tools, and media.
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Works on downward displacement of air.
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Ensures aseptic laboratory conditions.